Syringe method for stepwise chemical synthesis of oligonucleotides

Syringe method for stepwise chemical synthesis of oligonucleotides.

A simple procedure is described for synthesis of oligonucleotides by phosphate chemistry. Chains can be constructed rapidly with minimal equipment (a syringe and reagent bottles). The method is illustrated by synthesis of d-TGCAGGTT. Pertinent supporting data on the effect of variations in the detritylation, condensation, oxidation, capping and cleavage steps in the synthetic approach and in is...

Chemical Synthesis of Oligonucleotides

It can fairly be said that synthetic oligonucleotides are the fuel that drives the engine of molecular biology. Nearly every technique in use today in molecular biology employs chemically synthesized DNAs or RNAs. This includes PCR, Real-Time PCR, DNA sequencing, site directed mutagenesis, single-nucleotide polymorphism (SNP) assays, microarrays, and the rapidly expanding world of small RNAs. U...

Chemical Synthesis and Purification of Oligonucleotides

Simple chemical method for synthesis of CuO/CuI nanocomposite

This study reports the simple process to synthesis of CuO/CuI nanocomposite in a non-aqueous solution. In the conducted experiments CuI and Dimethyl Sulfoxide (DMSO) were used as the precursors. Moreover, DMSO had role of solvent and ligand simultaneously. In experiments the effects of oleic acid as the particles size modifier, temperature and concentration were investigated. Crystalline struct...

Simple chemical method for synthesis of CuO/CuI nanocomposite

This study reports the simple process to synthesis of CuO/CuI nanocomposite in a non-aqueous solution. In the conducted experiments CuI and Dimethyl Sulfoxide (DMSO) were used as the precursors. Moreover, DMSO had role of solvent and ligand simultaneously. In experiments the effects of oleic acid as the particles size modifier, temperature and concentration were investigated. Crystalline struct...